Actin filament attachments for sustained motility in vitro are maintained by filament bundling.
We reconstructed cellular motility in vitro from individual proteins to investigate how actin filaments are organized at the leading puddles the platypus chocolate edge.Using total internal reflection fluorescence microscopy of actin filaments, we tested how profilin, Arp2/3, and capping protein (CP) function together to propel thin glass nanofiber